I-Diphenylcarbodiimide, ifomula yamakhemikhali2162-74-5, iyinhlanganisela eye yadonsa ukunaka okusabalele emkhakheni wamakhemikhali ezinto eziphilayo. Inhloso yalesi sihloko ukuhlinzeka ngesifinyezo se-diphenylcarbodiimide, izici zayo, ukusetshenziswa, kanye nokubaluleka kwezinhlelo zokusebenza ezihlukahlukene.

I-Diphenylcarbodiimide iyinhlanganisela ene-molecular formula C13H10N2. I-crystalline emhlophe ukuya kokumhlophe eqinile, inyibilika kancane emanzini, incibilika kalula ku-acetone, i-ethanol, i-chloroform nezinye izincibilikisi eziphilayo. Le nhlanganisela yaziwa kakhulu ngekhono layo lokusebenza njenge-reagent eguquguqukayo ku-organic synthesis, ikakhulukazi ekwakhekeni kwama-amide nama-urea.

Enye yezinto ezibalulekile ze-diphenylcarbodiimide ukuphinda isebenze nama-amine nama-carboxylic acid, okuholela ekwakhekeni kwama-amide. Lokhu kusabela kubizwa ngokuthi i-carbodiimide coupling reaction futhi isetshenziswa kakhulu ekuhlanganiseni kwe-peptide nokuguqulwa kwe-biomolecule. Ngaphezu kwalokho, i-diphenylcarbodiimide ingasabela ngotshwala ukuze yenze i-polyurethane, iyenze ibe i-reagent ebalulekile ekukhiqizeni izinto ze-polyurethane.

Embonini yezemithi, i-diphenylcarbodiimide ingasetshenziswa ukuze kuhlanganiswe imithi ehlukahlukene kanye nezimaphakathi zemithi. Ikhono layo lokukhuthaza ukwakheka kwebhondi ye-amide libaluleke kakhulu ekuthuthukisweni kwezidakamizwa ze-peptide nama-bioconjugates. Ngaphezu kwalokho, ukusebenza kabusha kwenhlanganisela kuma-carboxylic acid kuyenza ibe ithuluzi eliwusizo lokuhlanganisa izidakamizwa kuma-molecule aqondiswe kuwo, ngaleyo ndlela kuvumela ukuklanywa kwezinhlelo zokulethwa kwezidakamizwa okuhlosiwe.

Ngaphezu kwendima yawo ekuhlanganiseni kwezinto eziphilayo, i-diphenylcarbodiimides iye yacwaningwa ukuze isetshenziswe okungenzeka kusayensi yezinto ezibonakalayo. Ukusebenzisana kabusha kwe-compound ku-alcohols kukwenza kube usizo ekukhiqizeni amagwebu e-polyurethane, okokunamathela kanye nezinamatheliso. Ikhono layo lokwenza i-polyurethane liyenza ibe isithako esibalulekile ekwakhiweni kwezinto ezihlala isikhathi eside, eziguquguqukayo ze-polyurethane ezisetshenziswa ezinhlobonhlobo zezimboni kusukela ekwakhiweni kuya kwezezimoto.

Ukubaluleka kwe-diphenylcarbodiimides kudlulela emikhakheni ye-bioconjugation kanye ne-bioorthogonal chemistry. Ukusebenza kabusha kwayo kuma-biomolecules kusetshenziselwe ukuguqulwa kwesayithi okuqondene namaprotheni nama-nucleic acid, okuvumela ukuthuthukiswa kwamanoveli ama-bioconjugate kanye nama-bioimaging probe. Ngaphezu kwalokho, ukuhambisana kwenhlanganisela nezindawo ezinamanzi kuyenza ibe yithuluzi elibalulekile lokuthuthukisa ukusabela kwe-bioorthogonal ukutadisha izinqubo zebhayoloji ezinhlelweni eziphilayo.

Kafushane, i-diphenylcarbodiimide, ifomula yamakhemikhali 2162-74-5, iyinhlanganisela esebenza ngezindlela eziningi enezinhlelo zokusebenza ezihlukahlukene emkhakheni we-organic synthesis, imithi, isayensi yezinto ezibonakalayo, kanye ne-bioconjugated chemistry. Ukusebenza kabusha kwayo kuma-amine, ama-carboxylic acid, nama-alcohol kuyenza ibe into ebalulekile ekwakhekeni kwama-amide, ama-carbamates, nama-bioconjugates. Njengoba ucwaningo kulezi zindawo luqhubeka nokuthuthuka, i-diphenylcarbodiimides kungenzeka ihlale ibamba iqhaza elibalulekile ekuthuthukisweni kwezinto ezintsha nezinhlanganisela ze-bioactive, okunomthelela entuthukweni emikhakheni eyahlukene yesayensi nezimboni.